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KMID : 0381920130430010001
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Korean Journal of Microscopy
2013 Volume.43 No. 1 p.1 ~ p.8
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Improvement of Alignment Accuracy in Electron Tomography
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Jou Hyeong-Tae
Lee Su-Jeong
Kim Han-Joon
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Abstract
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We developed an improved method for tilt series alignment with fiducial markers in electron tomography. Based on previous works regarding alignment, we adapted the Levenberg-Marquardt method to solve the nonlinear least squares problem by incorporating a new formula for the alignment model. We also suggested a new method to estimate the initial value for inversion with higher accuracy. The proposed approach was applied to geopolymers. A better alignment of the tilt series was achieved than that by IMOD S/W. The initial value estimation provided both stability and a good rate of convergence since the new method uses all marker positions, including those partly covering the tilt images.
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KEYWORD
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Electron tomography, Geopolymer, Alignment, Fiducial marker
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